LncRNA-MM2P Identified as a Modulator of Macrophage M2 Polarization
作者： Ji Cao , Rong Dong , Li Jiang , Yanling Gong , Meng Yuan
DOI: 10.1158/2326-6066.CIR-18-0145
关键词: Cell biology 、 Gene knockdown 、 Gene expression profiling 、 Cell 、 Chemistry 、 Carcinogenesis 、 M2 Macrophage 、 Phosphorylation 、 Transcriptome 、 Downregulation and upregulation
摘要: M2 polarization of macrophages is essential for their function in immunologic tolerance, which might promote tumorigenesis. However, the molecular mechanism behind process not fully understood. Given that several lines evidence have suggested long noncoding RNAs (lncRNAs) could be involved regulating immune cell differentiation and function, current study aimed to identify lncRNAs specifically modulate macrophage polarization. By utilizing a series cell-based models, total 25 with altered expression were documented based on lncRNA microarray-based profiling assays. Among them, lncRNA-MM2P was only upregulated during but downregulated M1 macrophages. Knockdown blocked cytokine-driven weakened angiogenesis-promoting feature by reducing phosphorylation STAT6. Moreover, manipulating impaired macrophage-mediated promotion tumorigenesis, tumor growth vivo, angiogenesis. Collectively, our identifies as modulator required uncovers its role macrophage-promoted
aacrjournals.org
sci-hub.se 参考文章(42)
Keshav Raj Sigdel, Ao Cheng, Yin Wang, Lihua Duan, YanLin Zhang, The Emerging Functions of Long Noncoding RNA in Immune Cells: Autoimmune Diseases Clinical & Developmental Immunology. ,vol. 2015, pp. 848790- 848790 ,(2015) , 10.1155/2015/848790
Sugata Roy, Sebastian Schmeier, Erik Arner, Tanvir Alam, Suraj P Parihar, Mumin Ozturk, Ousman Tamgue, Hideya Kawaji, Michiel JL de Hoon, Masayoshi Itoh, Timo Lassmann, Piero Carninci, Yoshihide Hayashizaki, Alistair RR Forrest, Vladimir B Bajic, Reto Guler, FANTOM Consortium, Frank Brombacher, Harukazu Suzuki, None, Redefining the transcriptional regulatory dynamics of classically and alternatively activated macrophages by deepCAGE transcriptomics Nucleic Acids Research. ,vol. 43, pp. 6969- 6982 ,(2015) , 10.1093/NAR/GKV646
Paola Allavena, Antonio Sica, Cecilia Garlanda, Alberto Mantovani, The Yin-Yang of tumor-associated macrophages in neoplastic progression and immune surveillance Immunological Reviews. ,vol. 222, pp. 155- 161 ,(2008) , 10.1111/J.1600-065X.2008.00607.X
Minna U. Kaikkonen, Nathanael J. Spann, Sven Heinz, Casey E. Romanoski, Karmel A. Allison, Joshua D. Stender, Hyun B. Chun, David F. Tough, Rab K. Prinjha, Christopher Benner, Christopher K. Glass, Remodeling of the Enhancer Landscape during Macrophage Activation Is Coupled to Enhancer Transcription Molecular Cell. ,vol. 51, pp. 310- 325 ,(2013) , 10.1016/J.MOLCEL.2013.07.010
Martin Franz Sprinzl, Andreas Puschnik, Anna Melissa Schlitter, Arno Schad, Kerstin Ackermann, Irene Esposito, Hauke Lang, Peter Robert Galle, Arndt Weinmann, Mathias Heikenwälder, Ulrike Protzer, Sorafenib inhibits macrophage-induced growth of hepatoma cells by interference with insulin-like growth factor-1 secretion. Journal of Hepatology. ,vol. 62, pp. 863- 870 ,(2015) , 10.1016/J.JHEP.2014.11.011
Dao-Feng Ben, Xi-Ya Yu, Guang-Yu Ji, De-Yi Zheng, Kai-Yang Lv, Bing Ma, Zhao-Fan Xia, TLR4 mediates lung injury and inflammation in intestinal ischemia-reperfusion. Journal of Surgical Research. ,vol. 174, pp. 326- 333 ,(2012) , 10.1016/J.JSS.2010.12.005
Sofia Edin, Maria L. Wikberg, Anna M. Dahlin, Jörgen Rutegård, Åke Öberg, Per-Arne Oldenborg, Richard Palmqvist, The Distribution of Macrophages with a M1 or M2 Phenotype in Relation to Prognosis and the Molecular Characteristics of Colorectal Cancer PLoS ONE. ,vol. 7, pp. e47045- ,(2012) , 10.1371/JOURNAL.PONE.0047045
Thomas Thum, Noncoding RNAs and myocardial fibrosis Nature Reviews Cardiology. ,vol. 11, pp. 655- 663 ,(2014) , 10.1038/NRCARDIO.2014.125
J. Weischenfeldt, B. Porse, Bone Marrow-Derived Macrophages (BMM): Isolation and Applications CSH Protocols. ,vol. 2008, ,(2008) , 10.1101/PDB.PROT5080
Toby Lawrence, Gioacchino Natoli, Transcriptional regulation of macrophage polarization: enabling diversity with identity Nature Reviews Immunology. ,vol. 11, pp. 750- 761 ,(2011) , 10.1038/NRI3088