作者: Claudio Sorio , Giuseppe Bellisola
DOI:
关键词: Microscopy 、 Label free 、 Instrumentation (computer programming) 、 Analytical technique 、 Cancer research 、 Infrared spectroscopy 、 Scale (chemistry) 、 Spectral data 、 Non destructive 、 Computer science
摘要: Since the middle of 20th century infrared (IR) spectroscopy coupled to microscopy (IR microspectroscopy) has been recognized as a non destructive, label free, highly sensitive and specific analytical method with many potential useful applications in different fields biomedical research particular cancer diagnosis. Although technological improvements have made facilitate this powerful technique, it not yet properly come into scientific background end users. Therefore, achieve those fundamental objectives an interdisciplinary approach is needed basic scientists, spectroscopists, biologists clinicians who must effectively communicate understand each other's requirements challenges. In review we aim at illustrating some principles Fourier transform (FT) Infrared vibrational (microFT-IR) interrogate molecules specimen by mid-IR radiation. Penetrating basics molecular vibrations might help us whether, when how complementary information obtained microFT-IR could become our and/or diagnostic activities. MicroFT-IR techniques allowing acquire about composition structure sample within micrometric scale matter seconds will be illustrated well limitations discussed. How biochemical, structural, dynamical systems can bench top instrumentation also presented together methods treat interpret IR spectral data applicative examples. The absorbance spectrum one most information-rich concise way represent whole “… omics” cell and, such, fits all characteristics for development clinically biomarker.