Antigenic phenotype of lung carcinomas: usual spectrum of distribution of thyroid transcription factor-1, cytokeratin 7, cytokeratin 20, and neuron specific enolase--basic immunohistochemical study of 21 cases.

摘要: Abstract (Full text is available at http://www.manu.edu.mk/prilozi). Immunohistochemistry (IHC), as such, can be used in routine pathology order to make correct diagnosis of lung carcinomas. Consequently, more detailed analyses are needed this field a wide spectrum unique combinations for such pulmonary neoplasms. Our aim was apply an antibody panel, and examine confirm its utility the differential cancer. Twenty-one cases (both bioptic surgical material) diagnosed cancer were investigated. An immunohistochemical analysis - (RTU FLEX Immunoperoxidase system) made using Dako monoclonal antibodies (Cytokeratin 7, CK7; Cytokeratin 20, CK20; Neuron specific enolase, NSE, Thyroid transcription factor-1, TTF1 Leucocyte common antigen, LCA). LCA expression not expressed any our cases. Most adenocarcinoma CK7(+) 83.3% TTF1(±) 50%. The CK20(+) showed metastatic deposit lung. TTF1(+) 100%, NSE(+) 100% CK7(-) 66.66% found most SCLC. -100% had highest carcionoid tumour, while For squamous cell carcinoma (SqCC), immunostaining negative except focal weak NSE 60%, so we did some extra IHC CKHMW antibody, which expression. essential panel that have confirmed suggest basic neoplasms is: TTF1, CK7, CK20 NSE. Due high number co-occurrunces should performed alone, but integrated conjunction with morphological diagnosis. Key words: immunohistochemistry, neoplasms, antigen distribution, panel.