Investigation of TEMPO partitioning in different skin models as measured by EPR spectroscopy - Insight into the stratum corneum
作者： Anja Elpelt , Daniela Ivanov , Anna Nováčková , Andrej Kováčik , Michaela Sochorová
DOI: 10.1016/J.JMR.2019.106637
关键词: Biophysics 、 Epidermis 、 Spin probe 、 Membrane 、 Infrared spectroscopy 、 Electron paramagnetic resonance 、 Ex vivo 、 Stratum corneum 、 Chemistry 、 Dermis
摘要: Electron paramagnetic resonance (EPR) spectroscopy represents an established tool to study properties of microenvironments, e.g. investigate the structure and dynamics biological artificial membranes. In this study, partitioning spin probe 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) in ex vivo human abdominal breast skin, porcine ear skin as well normal inflammatory vitro equivalents was investigated by EPR spectroscopy. Furthermore, stratum corneum (SC) lipid composition (as determined high-performance thin-layer chromatography), SC chain order (probed infrared spectroscopy) thickness (investigated histology) were models. X-band measurements have shown that TEMPO partitions lipophilic hydrophilic microenvironment varying ratios different Ex exhibited highest amount microenvironment. contrast, lowest equivalents. Individual spectra epidermis including dermis indicated mainly corresponds most part epidermis, SC. The independent but correlated with thickness. conclusion, could be a novel technique determine differences thickness, thus suitably complementing existing methods.
harvard.edu
sci-hub.se 参考文章(67)
Julia Caussin, Gert S. Gooris, Michelle Janssens, Joke A. Bouwstra, Lipid organization in human and porcine stratum corneum differs widely, while lipid mixtures with porcine ceramides model human stratum corneum lipid organization very closely. Biochimica et Biophysica Acta. ,vol. 1778, pp. 1472- 1482 ,(2008) , 10.1016/J.BBAMEM.2008.03.003
Barbora Školová, Klára Hudská, Petra Pullmannová, Andrej Kováčik, Karel Palát, Jaroslav Roh, Jana Fleddermann, Irina Estrela-Lopis, Kateřina Vávrová, Different Phase Behavior and Packing of Ceramides with Long (C16) and Very Long (C24) Acyls in Model Membranes: Infrared Spectroscopy Using Deuterated Lipids Journal of Physical Chemistry B. ,vol. 118, pp. 10460- 10470 ,(2014) , 10.1021/JP506407R
JL Rees, K Robertson, Variation in Epidermal Morphology in Human Skin at Different Body Sites as Measured by Reflectance Confocal Microscopy Acta Dermato-venereologica. ,vol. 90, pp. 368- 373 ,(2010) , 10.2340/00015555-0875
W. L. Hubbell, H. M. McConnell, Spin-label studies of the excitable membranes of nerve and muscle. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 61, pp. 12- 16 ,(1968) , 10.1073/PNAS.61.1.12
Kateřina Vávrová, Dominika Henkes, Kay Strüver, Michaela Sochorová, Barbora Školová, Madeleine Y. Witting, Wolfgang Friess, Stephan Schreml, Robert J. Meier, Monika Schäfer-Korting, Joachim W. Fluhr, Sarah Küchler, Filaggrin Deficiency Leads to Impaired Lipid Profile and Altered Acidification Pathways in a 3D Skin Construct Journal of Investigative Dermatology. ,vol. 134, pp. 746- 753 ,(2014) , 10.1038/JID.2013.402
S.F. Haag, B. Taskoparan, R. Bittl, C. Teutloff, R. Wenzel, A. Fahr, M. Chen, J. Lademann, M. Schäfer-Korting, M.C. Meinke, Stabilization of Reactive Nitroxides Using Invasomes to Allow Prolonged Electron Paramagnetic Resonance Measurements Skin Pharmacology and Physiology. ,vol. 24, pp. 312- 321 ,(2011) , 10.1159/000330235
Richard Mendelsohn, David J. Moore, Vibrational spectroscopic studies of lipid domains in biomembranes and model systems Chemistry and Physics of Lipids. ,vol. 96, pp. 141- 157 ,(1998) , 10.1016/S0009-3084(98)00085-1
S. Nicoli, C. Padula, V. Aversa, B. Vietti, P.W. Wertz, A. Millet, F. Falson, P. Govoni, P. Santi, Characterization of Rabbit Ear Skin as a Skin Model for in vitro Transdermal Permeation Experiments: Histology, Lipid Composition and Permeability Skin Pharmacology and Physiology. ,vol. 21, pp. 218- 226 ,(2008) , 10.1159/000135638
Jürgen Fuchs, Rolf J. Mehlhorn, Lester Packer, Free radical reduction mechanisms in mouse epidermis skin homogenates Journal of Investigative Dermatology. ,vol. 93, pp. 633- 640 ,(1989) , 10.1111/1523-1747.EP12319780
Jürgen Fuchs, Norbert Groth, Thomas Herrling, Guido Zimmer, Electron Paramagnetic Resonance Studies on Nitroxide Radical 2,2,5,5-Tetramethyl-4-piperidin-1-oxyl (TEMPO) Redox Reactions in Human Skin Free Radical Biology and Medicine. ,vol. 22, pp. 967- 976 ,(1997) , 10.1016/S0891-5849(96)00433-9