Expression of the Na-K-2Cl Cotransporter in Branchial Mitochondrion- Rich Cells of Mozambique Tilapia (Oreochromis mossambicus) Subjected to Varying Chloride Conditions
作者： Li Yih Lin , Jiun-Lin Horng
DOI:
关键词: Oreochromis mossambicus 、 Membrane 、 Endocrinology 、 Molecular biology 、 Internal medicine 、 Tilapia 、 Immunocytochemistry 、 Biology 、 Confocal microscopy 、 Osmoregulation 、 Cotransporter 、 Staining
摘要: The Na/K/2Cl cotransporter (NKCC) plays a critical role of active Cl(superscript -) transport through the basolateral membranes mitochondrion-rich (MR) cells in seawater teleosts. Recently, NKCC was also found apical MR freshwater (FW)-acclimated tilapia (Oreochromis mossambicus). In this study, immunocytochemistry and immunoblotting were used to analyze expression levels FW-acclimated response different ambient chloride levels. amount branchial from low-Cl(superscript -)- (0.002-0.006 mM)-acclimated about 3-and 5.5-fold higher than that normal-(0.49-0.51 mM) high-Cl(superscript (9.8-10.2 tilapia. With confocal microscopy, localized mainly with -)-dependent abundances. staining intensity individual significantly group normal groups. Results suggest apically is induced by deficiency probably involved uptake mechanism cells.
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