Purification and Characterization of Recombinant Anthrax Edema Factor
作者： Yu-Gene Kim
DOI: 10.9766/KIMST.2011.14.4.710
关键词: Edema 、 Enzyme assay 、 Biochemistry 、 Anthrax toxin 、 Bacillus anthracis 、 Intracellular 、 Chemistry 、 Recombinant DNA 、 Cloning 、 Anthrax edema factor
摘要: Edema factor(EF) is a portion of anthrax toxin which produces edema when combined with protective antigen. This paper describes about technique for cloning, expression, purification and activity test EF. Using the E. coli expression system, we could make recombinant EF protein although it`s origin Bacillus anthracis. And also culture massively purify highly pure protein. Finally confirm enzyme purified to increase intracellular cAMP level. Through establishing this technique, it can be possible research in depth apply many other biology.
koreascience.or.kr参考文章(22)
Stephen H. Leppla, Purification and characterization of adenylyl cyclase from Bacillus anthracis. Methods in Enzymology. ,vol. 195, pp. 153- 168 ,(1991) , 10.1016/0076-6879(91)95162-D
M T Tippetts, D L Robertson, Molecular cloning and expression of the Bacillus anthracis edema factor toxin gene: a calmodulin-dependent adenylate cyclase. Journal of Bacteriology. ,vol. 170, pp. 2263- 2266 ,(1988) , 10.1128/JB.170.5.2263-2266.1988
A M Friedlander, Macrophages are sensitive to anthrax lethal toxin through an acid-dependent process. Journal of Biological Chemistry. ,vol. 261, pp. 7123- 7126 ,(1986) , 10.1016/S0021-9258(17)38364-3
Andreas Gille, Gerald H. Lushington, Tung-Chung Mou, Michael B. Doughty, Roger A. Johnson, Roland Seifert, Differential inhibition of adenylyl cyclase isoforms and soluble guanylyl cyclase by purine and pyrimidine nucleotides Journal of Biological Chemistry. ,vol. 279, pp. 19955- 19969 ,(2004) , 10.1074/JBC.M312560200
Donald L. Robertson, Stephen H. Leppla, Molecular cloning and expression in Escherichia coli of the lethal factor gene of Bacillus anthracis Gene. ,vol. 44, pp. 71- 78 ,(1986) , 10.1016/0378-1119(86)90044-2
Yuequan Shen, Natalia L Zhukovskaya, Qing Guo, Jan Florián, Wei-Jen Tang, Calcium-independent calmodulin binding and two-metal-ion catalytic mechanism of anthrax edema factor. The EMBO Journal. ,vol. 24, pp. 929- 941 ,(2005) , 10.1038/SJ.EMBOJ.7600574
Andrew D. Pannifer, Thiang Yian Wong, Robert Schwarzenbacher, Martin Renatus, Carlo Petosa, Jadwiga Bienkowska, D. Borden Lacy, R. John Collier, Sukjoon Park, Stephen H. Leppla, Philip Hanna, Robert C. Liddington, Crystal structure of the anthrax lethal factor Nature. ,vol. 414, pp. 229- 233 ,(2001) , 10.1038/N35101998
Carlo Petosa, R. John Collier, Kurt R. Klimpel, Stephen H. Leppla, Robert C. Liddington, Crystal structure of the anthrax toxin protective antigen. Nature. ,vol. 385, pp. 833- 838 ,(1997) , 10.1038/385833A0
Deliang Chen, Milind Misra, Laurie Sower, Johnny W. Peterson, Glen E. Kellogg, Catherine H. Schein, Novel inhibitors of anthrax edema factor Bioorganic & Medicinal Chemistry. ,vol. 16, pp. 7225- 7233 ,(2008) , 10.1016/J.BMC.2008.06.036
Jørgen Wesche, Jennifer L. Elliott, Pål Ø. Falnes, Sjur Olsnes, R. John Collier, Characterization of Membrane Translocation by Anthrax Protective Antigen Biochemistry. ,vol. 37, pp. 15737- 15746 ,(1998) , 10.1021/BI981436I