Development and Characterization of Somatic Hybrids of Ulva reticulata Forsskål (×) Monostroma oxyspermum (Kutz.)Doty
作者： Vishal Gupta , Puja Kumari , CRK Reddy
关键词: DNA profiling 、 Stearidonic acid 、 Introgression 、 Amplified fragment length polymorphism 、 Protoplast 、 Hybrid 、 Botany 、 Monostroma 、 Genetics 、 Biology 、 Linoleic acid
摘要: Ulvophycean species with diverse trait characteristics provide an opportunity to create novel allelic recombinant variants. The present study reports the development of seaweed variants improved agronomic traits through protoplast fusion between Monostroma oxyspermum (Kutz.) Doty and Ulva reticulata Forsskal. A total 12 putative hybrids were screened based on variations in morphology DNA content over partners. DNA-fingerprinting by inter simple sequence repeat (ISSR) amplified fragment length polymorphism (AFLP) analysis confirmed genomic introgression hybrids. fingerprint revealed sharing parental alleles regenerated a few that unique epigenetic estimated terms methylation also loci both functional for growth showed hybrid heterotic (DGR%= 36.7±1.55%) partners U. (33.2±2.6%) M. (17.8±1.77%), while others superior mid-parental value (25.2±2.2%) (p<0.05). fatty acid (FA) notable Most increased polyunsaturated FAs (PUFAs) compared saturated (SFAs) mainly includes nutritionally important linoleic acid, α-linolenic oleic stearidonic docosahexaenoic acid. other differences observed include cellulose antioxidative potential varieties developed this unequivocally demonstrate significance technique developing varients macroalgae.
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