Differential Expression of CD180 and sIgM by B Chronic Lymphocytic Leukaemia Cells (B-CLL) Using Mutated and Unmutated IgVh Genes.

摘要: Introduction. B cell chronic lymphocytic leukaemia (B-CLL) is a heterogeneous disease as shown by differential expression of variety surface and cytoplasmic markers. In search for markers that could define biological activity different B-CLL subsets, we have studied the Toll-like receptor (TLR) family member CD180 in relation to other mutation status IgVh genes. Methods. Seventy eight patients (68 untreated 10 treated) 15 age-matched controls were three clinics. CD19+ cells stained using indirect immuno fluorescence CD180, IgM (sIgM), CD79b CD38, analysed flow cytometry data expressed relative antibody binding sites (RBS)/cell each marker. Monoclonal anti-CD5 antibodies also used with anti determine levels control CD5+ cells. was determined 47 patients. Results had variable expression, but this always less (1036 ± 935 RBS/cell) than normal blood (5548 2271 stable up 18 months. Significantly higher mutated (M) compared those unmutated (UM) genes. This contrast sIgM UM M genes (Figure). Conclusions. at on level which versus genes. This supports notion represent population actively responding signals (perhaps self antigens) via their IgM. ![Figure][1] Figure [1]: pending:yes