An assessment of the cage-culture turbidostat as an alternative algal bioassay

作者: N. Clarkson , J.W. Leftley , D.T. Meldrum , J.W. Watson

DOI: 10.1016/S0043-1354(97)00302-3

关键词: ToxicityDCMUBiologyCage cultureTurbidostatGrowth rateBotanyBioassayPhaeodactylum tricornutumEffluentChromatography

摘要: Abstract A computer controlled cage-culture turbidostat (CCT) is described as an alternative to batch algal bioassays. The CCT designed monitor growth changes in response the addition of toxicants including chronic toxic effects comparison culture toxicity tests which are most successful assessing acute responses. system easily and quickly set up, with minimum operator time required once assembled. alga Phaeodactylum tricornutum (CCAP 1052/6) was used test species. Stable could be maintained for 14 days, sufficient run bioassay experiments using both herbicide Diuron ™ (DCMU) a complex industrial effluent. Additions various concentrations toxins chamber caused rate chl- concentration culture. Median Inhibitory Concentration effluent calculated cause 50% reduction (IC 50 ) estimated 0.33  μ M DCMU, 15.33% Comparable results were obtained when standard inhibition (AGIT) test, 72 h-IC values 0.09  DCMU 16.4% This study indicates potential use water quality monitoring, may provide more environmentally relevant information than commonly AGIT test.

参考文章(18)
Olav Skipnes, Ingvar Eide, Arne Jensen, Cage Culture Turbidostat: a Device for Rapid Determination of Algal Growth Rate Applied and Environmental Microbiology. ,vol. 40, pp. 318- 325 ,(1980) , 10.1128/AEM.40.2.318-325.1980
W. E. Miller, S. A. Peterson, J. C. Greene, C. A. Callahan, Comparative Toxicology of Laboratory Organisms for Assessing Hazardous Waste Sites Journal of Environmental Quality. ,vol. 14, pp. 569- 574 ,(1985) , 10.2134/JEQ1985.00472425001400040019X
J. A. van Heever, J. U. Grobbelaar, Evaluation of a short-incubation-time small-volume radiocarbon-uptake algal toxicity test Journal of Applied Phycology. ,vol. 8, pp. 65- 71 ,(1996) , 10.1007/BF02186224
J. Řičica, Continuous cultivation of microorganisms Folia Microbiologica. ,vol. 13, pp. 389- 415 ,(1970) , 10.1007/BF02875760
Kjetill Ostgaard, Arne Jensen, Anders Johnsson, Lithium ions lengthen the circadian period of growing cultures of the diatom Skeletonema costatum Physiologia Plantarum. ,vol. 55, pp. 285- 288 ,(1982) , 10.1111/J.1399-3054.1982.TB00293.X
Peter J. Wangersky, Regine L. Maass, Bioavailability: the organism as sensor Marine Chemistry. ,vol. 36, pp. 199- 213 ,(1991) , 10.1016/S0304-4203(09)90062-7
D. St-Laurent, C. Blaise, P. Macquarrie, R. Scroggins, B. Trottier, Comparative assessment of herbicide phytotoxicity to Selenastrum capricornutum using microplate and flask bioassay procedures Environmental Toxicology & Water Quality. ,vol. 7, pp. 35- 48 ,(1992) , 10.1002/TOX.2530070104
Niels Nyholm, Expression of results from growth inhibition toxicity tests with algae Archives of Environmental Contamination and Toxicology. ,vol. 19, pp. 518- 522 ,(1990) , 10.1007/BF01059070