Characterization of the protein encoded by gene UL49A of herpes simplex virus type 1.

摘要: Herpes simplex virus type 1 (HSV-1) gene UL49A potentially encodes a primary translation product of 91 residues with signal sequence at the N terminus and membrane anchor domain near C terminus. Mutants were generated in this utilized to characterize encoded protein on SDS-PAGE as 6.7 kDa species which fractionated infected cell membranes, was relatively abundant virion component, not detectably O-glycosylated. The identified by microsequencing 68 residue polypeptide formed removal 23 from product. Cleavage also demonstrated vitro transcription presence microsomal membranes. efficiently solubilized along envelope proteins treatment virions non-ionic detergent but only reducing agent, suggesting that it may be an is disulphide-linked tegument. It apparent mutational analysis 10 amino acid are essential for synthesis protein, cleavage, targeting membranes virions, linkage tegument growth culture.