In vivo evaluation of mutagenic and recombinagenic activities of Brazilian propolis.

作者: Carmem Regine Faleiro Rodrigues , Luciana Ciarelli Plentz , Maria Cristina Marcucci , Rafael Rodrigues Dihl , Mauricio Lehmann

DOI: 10.1016/J.FCT.2016.07.037

关键词: ToxicologyArtepillin CIn vivoFood scienceCyp450 enzymesBiologySomatic recombinationPolyphenolGenotoxicityPropolis

摘要: Propolis is a resinous, complex mixture of compounds collected by the bee species Apis mellifera. This study investigated genotoxicity green and brown propolis in southeast Brazil using somatic mutation recombination test (SMART) Drosophila melanogaster. The effect five concentrations (0.5, 1.0, 2.0, 4.0, 7.5 mg/mL) both types was analyzed standard (ST) high-bioactivation (HB) crosses, which have normal high levels cytochrome P450 enzymes, respectively. results show that evaluated no mutagenic action, either cross. Moreover, chromatography findings revealed different chemical compositions. Brown had lower polyphenols (∼7.2 mg/mL), compared to type (34.9 mg/g). Taken together, present literature reports point safety consuming low amounts propolis, considering risk genetic damage, confirm absence recombinagenic actions investigated.

参考文章(52)
Maria Cristina Marcucci, Federico Ferreres, Angela Ramalho Custódio, Marcia M.C. Ferreira, Vassya Stefanova Bankova, Cristina García-Viguera, Walter Antonio Bretz, Evaluation of phenolic compounds in Brazilian propolis from different geographic regions. Zeitschrift für Naturforschung C. ,vol. 55, pp. 76- 81 ,(2000) , 10.1515/ZNC-2000-1-215
Ingridy Simone Ribeiro Cabral, Tatiane Luiza Cadorin Oldoni, Severino Matias de Alencar, Pedro Luiz Rosalen, Masaharu Ikegaki, The correlation between the phenolic composition and biological activities of two varieties of Brazilian propolis (G6 and G12) Brazilian Journal of Pharmaceutical Sciences. ,vol. 48, pp. 557- 564 ,(2012) , 10.1590/S1984-82502012000300023
Yoshimi Nakajima, Kazuhiro Tsuruma, Masamitsu Shimazawa, Satoshi Mishima, Hideaki Hara, Comparison of bee products based on assays of antioxidant capacities BMC Complementary and Alternative Medicine. ,vol. 9, pp. 4- 4 ,(2009) , 10.1186/1472-6882-9-4
Ewelina Szliszka, Alicja Z. Kucharska, Anna Sokół-Łętowska, Anna Mertas, Zenon P. Czuba, Wojciech Król, Chemical Composition and Anti-Inflammatory Effect of Ethanolic Extract of Brazilian Green Propolis on Activated J774A.1 Macrophages. Evidence-based Complementary and Alternative Medicine. ,vol. 2013, pp. 976415- 976415 ,(2013) , 10.1155/2013/976415
Fábio Henrique Fernandes, Zaira da Rosa Guterres, Walmir Silva Garcez, Sávio Mestre Lopes, Joaquim Corsino, Fernanda Rodrigues Garcez, Assessment of the (anti)genotoxicity of brown propolis extracts from Brazilian Cerrado biome in a Drosophila melanogaster model Food Research International. ,vol. 62, pp. 20- 26 ,(2014) , 10.1016/J.FOODRES.2014.02.029
S.K. Abraham, U. Graf, Protection by coffee against somatic genotoxicity in drosophila: Role of bioactivation capacity Food and Chemical Toxicology. ,vol. 34, pp. 1- 14 ,(1996) , 10.1016/0278-6915(95)00087-9
Alexandra Christine Helena Franklan Sawaya, Ildenize Barbosa da Silva Cunha, Maria Cristina Marcucci, Analytical methods applied to diverse types of Brazilian propolis Chemistry Central Journal. ,vol. 5, pp. 27- 27 ,(2011) , 10.1186/1752-153X-5-27
Adrián Luis-Villaroya, Laura Espina, Diego García-Gonzalo, Susana Bayarri, Consuelo Pérez, Rafael Pagán, Bioactive properties of a propolis-based dietary supplement and its use in combination with mild heat for apple juice preservation International Journal of Food Microbiology. ,vol. 205, pp. 90- 97 ,(2015) , 10.1016/J.IJFOODMICRO.2015.03.020
Piotr Szweda, Katarzyna Gucwa, Ewelina Kurzyk, Ewa Romanowska, Katarzyna Dzierżanowska-Fangrat, Anna Zielińska Jurek, Piotr Marek Kuś, Sławomir Milewski, Essential Oils, Silver Nanoparticles and Propolis as Alternative Agents Against Fluconazole Resistant Candida albicans, Candida glabrata and Candida krusei Clinical Isolates Indian Journal of Microbiology. ,vol. 55, pp. 175- 183 ,(2015) , 10.1007/S12088-014-0508-2
Adriana Hitomi Matsuda, Ligia Bicudo de Almeida-Muradian, Validated method for the quantification of artepillin-C in Brazilian propolis Phytochemical Analysis. ,vol. 19, pp. 179- 183 ,(2008) , 10.1002/PCA.1043