Early expression of grain hardness in the developing wheat endosperm.

摘要: Seeds from near-isogenic hard and soft wheat lines were harvested at regular intervals 5 days post-anthesis to maturity examined for hardness using the single kernel characterisation system (SKCS). SKCS analysis revealed that could be distinguished 15 (dpa). This trend continued until where difference between was most marked. not applied small 5- 10-dpa kernels. Fresh developing endosperm material light microscopy no visible differences cultivars detected. When air-dried scanning electron (SEM) as early dpa. Accumulation of puroindoline a b investigated in seeds both Western blotting ELISA. Low levels detected cultivar 10 dpa, reaching maximum 32 In cultivar, negligible throughout grain development. Puroindoline accumulates but overall contents higher cultivar. These findings indicate is expressed very when few starch granules storage proteins deposited cells. Further, Heron differentiated by SEM stage development accumulation puroindolines methods used this study.