Corneal endothelial cell damage after experimental diode laser thermal keratoplasty.

摘要: PURPOSE To evaluate the safety of diode laser thermal keratoplasty (LTK) with respect to corneal endothelial cell damage. METHODS In an in vitro animal model system, porcine eyes were irradiated a continuously emitting at wavelengths (lambda) 1.85 or 1.87 microm, corresponding absorption coefficient (micro(a)) 1.1 2.0 mm(-1). Different irradiation and application parameters tested serially. determine temperature threshold for damage, buttons analyzed separately waterbath experiment. The damage was assessed after trypan blue alizarin red supravital staining under light microscopy. RESULTS thresholds 50% probability (ED50) determined temperatures 65 degrees C 10-second water-bath immersion, 59 60 seconds. Coagulations that reached deeper stromal layers revealed severe cellular alterations areas exposed Descemet's membrane. thermally induced changes dependent on power (wavelength). Mean diameter total 245 +/- 154 microm (range, 0 594 microm) maximal lateral extent by exposure diameter. Increasing decreased penetration depth irradiation, creating greater rise within stroma significantly less (P < .01), when same applied. calculated area paracentral human endothelium ranged from 1.8% 13.6%. CONCLUSION Data obtained this study transferred nomogram, demonstrating appropriate parameter improvements can minimize adverse effects endothelium. However, adjustment cornea indicates potential keratoplasty, should be considered performing elective procedure.