A two-step morphology-PCR strategy for the identification of nematode larvae recovered from muscles after artificial digestion at meat inspection.

摘要: To ensure that meat from livestock and game is safe for human consumption, European legislation lays down rules mandatory testing. Helminth larvae are a category of zoonotic foodborne pathogens can contaminate meat. Among helminths, the only nematode regulated in Europe regarding inspection Trichinella spp.. It precisely during testing other potentially be found. Due to current lack tools, their identification often very complicated. Nematode than Trichinella, recovered artificial digestions pig wild boar muscles France Germany, were subjected newly developed two-step scheme, which includes both morphological examination molecular assays. The first step general orientation towards broad taxonomic group; second consists targeted based on results step. Different parasites identified, some not such as Metastrongylus spp. Angiostrongylus vasorum, but others known Toxocara cati, Ascaris suum, Uncinaria stenocephala. strategy efficient could also applied sources.