Immunoelectron Microscopy: High-Resolution Immunocytochemistry

摘要: Subcellular structures as seen in the electron microscope require correlation to a biological function increase our knowledge about fundamental processes cell. Immunoelectron microscopy is tool map macromolecules these with high spatial resolution within context of Different methods sample preparation and immunolabeling strategies are available must be selected according question under investigation. Sample requires immobilization/fixation. This can achieved chemically followed by conventional or PLT embedding Tokuyasu cryosectioning. Alternatively, cryopreparation (high-pressure-freezing freeze-substitution resin at low temperature) applied. Basic preembedding labeling permeabilized fixed samples on-section which compatible different methods. Immunolabeling without thin sectioning performed whole mount freeze-fracture cytochemistry. Immunogold allows quantification stereological statistical evaluation.