The Use of Site-Directed Mutagenesis to Study GPCRs
作者： Alex C. Conner , James Barwell , David R. Poyner , Mark Wheatley
DOI: 10.1007/978-1-61779-126-0_5
关键词: Site-directed mutagenesis 、 Computational biology 、 Mutant Chimeric Proteins 、 Protein structure 、 Plasmid transfection 、 Effector 、 Bioinformatics 、 Function (biology) 、 G protein-coupled receptor 、 Mutagenesis (molecular biology technique) 、 Biology
摘要: G protein coupled receptors (GPCRs) are highly flexible and dynamic proteins, which able to interact with diverse ligands, effectors, regulatory proteins. Site-directed mutagenesis (SDM) is a powerful tool for providing insight into how these proteins actually work, both in its own right when used conjunction information provided by other techniques such as crystallography or molecular modelling. Mutagenesis has been identify characterise myriad of functionally important residues, motifs domains within the GPCR architecture, aspects similarity differences between major families GPCRs. This chapter presents necessary undertaking informative SDM Whilst this relevant structure/function studies -general, specific pitfalls protocols suited investigating GPCRs particular will be highlighted.
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