Investigation of the Prognostic Significance of Vasculogenic Mimicry and Its Inhibition by Sorafenib in Canine Mammary Gland Tumors.

摘要: Canine mammary gland tumor (CMT) is one of the most important tumors in intact female dogs, and due its similarity to human breast cancer (BC), it considered a model comparative oncology. A subset can show aggressive behavior, recurrent histological finding presence vasculogenic mimicry (VM). VM process which highly cells fuse, forming fluid-conducting channels without endothelial cells. Although, has been described canine inflammatory carcinoma, no previous studies have investigated prognostic predictive significance CMT. Thus, this research aimed investigate vivo capacity sorafenib inhibit vitro. was identified situ formalin-fixed paraffin-embedded CMT samples (n = 248) using CD31/PAS double staining. 33% (82/248). The more strongly related grade than subtype. Patients with positive experienced shorter survival times dogs (P < 0.0001). Due importance VEGF-A/VEGFR-2 autocrine feed-forward loop epithelial tumors, we association between VEGF-A VEGFR-2 expression by neoplastic associations or VM. Among VM-positive samples, all 82) showed high scores (3 4) for VEGFR-2, indicating that common overexpressing VEGFR-2. cultured two primary cell lines abilities (CM9 CM60) vitro evaluated anti-tumoral effect sorafenib. CM9 line half maximal inhibitory concentration (IC50) 2.61 μM, CM60 an IC50 1.34 μM. We performed assay treated each dose sorafenib, able Overall, our results indicated factor bearing had on