Pyrolytic Carbon Nanograss Enhances Neurogenesis and Dopaminergic Differentiation of Human Midbrain Neural Stem Cells.

摘要: Advancements in research on the interaction of human neural stem cells (hNSCs) with nanotopographies and biomaterials are enhancing ability to influence cell migration, proliferation, gene expression, tailored differentiation toward desired phenotypes. Here, fabrication pyrolytic carbon nanograss (CNG) is reported demonstrated that these can be employed as substrates boosting hNSCs into dopaminergic neurons (DAn), a long-time pursued goal regenerative medicine based replacement. In near future, such structures play crucial role future for stem-cell replacement therapy (CRT) bio-implants Parkinson's disease (PD). The unique combination randomly distributed topographies biocompatible material optimized provide suitable mechano-material cues adhesion, division, DAn midbrain hNSCs. results show presence biocoating poly-L-lysine (PLL), CNG enhances neurogenesis up 2.3-fold 3.5-fold. Moreover, first time, consistent evidence provided, CNGs without any PLL coating not only supporting survival but also lead significantly enhanced promote acquire phenotype compared coated topographies.