Effects of point mutations on formation and structure of the RNA primer for ColE1 DNA replication
作者： Hisao Masukata , Jun-ichi Tomizawa
DOI: 10.1016/0092-8674(84)90244-7
关键词: DNA replication 、 Biology 、 Suppressor mutation 、 Point mutation 、 Base pair 、 DNA 、 Primer (molecular biology) 、 Molecular biology 、 Primase 、 Transcription (biology) 、 General Biochemistry, Genetics and Molecular Biology
摘要: Abstract We report mutations affecting several steps of ColE1 primer formation. Primer precursor forms the hybrid with template DNA and is cleaved by RNAase H to form primer. Point at positions −264, −265, −268, −308 (base pairs upstream replication origin) reduce efficiency A suppressor mutation −18 increases for a mutant transcript reduces that wild-type transcript. Therefore, formation probably starts after transcription passes this position. −10 affects site cleavage H. The appears be determined distance from stem-loop structure immediately upstream. double −186 −188 results in an RNA secondary prevents use H-cleaved as
elsevier.com
sci-hub.se 参考文章(30)
Haruo Ohmori, Jun-ichi Tomizawa, Nucleotide sequence of the region required for maintenance of colicin E1 plasmid. Molecular Genetics and Genomics. ,vol. 176, pp. 161- 170 ,(1979) , 10.1007/BF00273210
Walter L. Staudenbauer, Structure and replication of the colicin E1 plasmid. Current Topics in Microbiology and Immunology. ,vol. 83, pp. 93- 156 ,(1978) , 10.1007/978-3-642-67087-9_3
Jun-ichi Tomizawa, Replication of Colicin El Plasmid DNA In Vitro Springer, Boston, MA. pp. 797- 826 ,(1978) , 10.1007/978-1-4684-0844-7_57
D J Donoghue, P A Sharp, Replication of colicin E1 plasmid DNA in vivo requires no plasmid-encoded proteins. Journal of Bacteriology. ,vol. 133, pp. 1287- 1294 ,(1978) , 10.1128/JB.133.3.1287-1294.1978
R F Fisher, C Yanofsky, Mutations of the beta subunit of RNA polymerase alter both transcription pausing and transcription termination in the trp operon leader region in vitro. Journal of Biological Chemistry. ,vol. 258, pp. 8146- 8150 ,(1983) , 10.1016/S0021-9258(20)82040-7
F J Kretschmer, A C Chang, S N Cohen, Indirect selection of bacterial plasmids lacking identifiable phenotypic properties. Journal of Bacteriology. ,vol. 124, pp. 225- 231 ,(1975) , 10.1128/JB.124.1.225-231.1975
Y. Sakakibara, J.-I. Tomizawa, Replication of colicin E1 plasmid DNA in cell extracts. II. Selective synthesis of early replicative intermediates. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 71, pp. 1403- 1407 ,(1974) , 10.1073/PNAS.71.4.1403
R. M. Lacatena, G. Cesareni, Base pairing of RNA I with its complementary sequence in the primer precursor inhibits ColE1 replication Nature. ,vol. 294, pp. 623- 626 ,(1981) , 10.1038/294623A0
Helen Donis-Keller, Site specific enzymatic cleavage of RNA Nucleic Acids Research. ,vol. 7, pp. 179- 192 ,(1979) , 10.1093/NAR/7.1.179
IGNACIO TINOCO, PHILIP N. BORER, BARBARA DENGLER, MARK D. LEVINE, OLKE C. UHLENBECK, DONALD M. CROTHERS, JAY GRALLA, Improved Estimation of Secondary Structure in Ribonucleic Acids Nature. ,vol. 246, pp. 40- 41 ,(1973) , 10.1038/NEWBIO246040A0