Molecular Analysis of "Fomitiporia mediterranea" Isolates from Esca-Affected Grapevines in Southern Italy

摘要: Summary. Four Fomitiporia isolates representative of a collection about 300 from esca-affected grapevines in southern Italy (Apulia, Campania and Abruzzo) were examined by molecular methods. The DNA each isolate was analysed means polymerase chain reaction (PCR amplification) using ITS5 ITS4 primers. An amplification product 740 bp obtained all isolates. denaturated products had the same migration pattern when single-strand conformation polymorphism. PCR fragments that included ribosomal ITS1-5.8S-ITS2 region sequenced. In order to ascertain taxonomic identity isolates, ITS sequences compared with those mediterranea, F. punctata robusta deposited GenBank. uniform homologous type culture mediterranea M. Fischer, which Italian compared.