1,25-dihydroxyvitamin D3 stimulates 45Ca2+-uptake by cultured vascular smooth muscle cells derived from rat aorta
作者： Tsutoma Inoue , Hiroyuki Kawashima
DOI: 10.1016/S0006-291X(88)80439-X
关键词: Calcitriol 、 Calcium ATPase 、 Calcium 、 Internal medicine 、 Cell culture 、 Receptor 、 Endocrinology 、 Vascular smooth muscle 、 Biology 、 Homeostasis 、 Cycloheximide
摘要: We have recently shown the presence of receptors for 1,25-dihydroxyvitamin D3 and that stimulates Ca-ATPase in vascular smooth muscle cells presumably via receptor mediated mechanism. These data suggest sterol may directly be involved regulation cellular calcium homeostasis. To further define action vitamin D cells, we studied effect on uptake calcium. stimulated 45Ca2+ by cultured A7r5, derived from fetal rat aorta, when were incubated with 18 hr. The was dose-dependent at 10(-10) to 10(-9) M, three orders magnitude higher concentration 25-hydroxyvitamin or 24,25-dihydroxyvitamin needed obtain similar effects. Furthermore, abolished cycloheximide (10(-5) M), a protein synthesis inhibitor. clearly regulate homeostasis
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