Evaluating and enhancing ribozyme reaction efficiency in mammalian cells

作者: Joshua T. Jones , Bruce A. Sullenger

DOI: 10.1038/NBT0997-902

关键词: RNA splicingRNACell biologyLigase ribozymeRibozymeMutationCellTransfectionMutantMolecular biologyBiology

摘要: … The trans-splicing ribozyme-3' exon transcript contains the L-21 version of the Tetrahymena group I intron” followed by a 3' exon sequence that consists of a 19 nucleotide spacer and …

参考文章(28)
Flossie Wong-Staal, Mang Yu, Eric Poeschla, Progress towards gene therapy for HIV infection. Gene Therapy. ,vol. 1, pp. 13- 26 ,(1994)
Jim Haseloff, Wayne L. Gerlach, Simple RNA enzymes with new and highly specific endoribonuclease activities Nature. ,vol. 334, pp. 585- 591 ,(1988) , 10.1038/334585A0
Klemens J. Hertel, Daniel Herschlag, Olke C. Uhlenbeck, A Kinetic and Thermodynamic Framework for the Hammerhead Ribozyme Reaction Biochemistry. ,vol. 33, pp. 3374- 3385 ,(1994) , 10.1021/BI00177A031
O. Elroy-Stein, B. Moss, Cytoplasmic expression system based on constitutive synthesis of bacteriophage T7 RNA polymerase in mammalian cells. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 87, pp. 6743- 6747 ,(1990) , 10.1073/PNAS.87.17.6743
Thomas R. Cech, Ribozymes and Their Medical Implications JAMA: The Journal of the American Medical Association. ,vol. 260, pp. 3030- 3034 ,(1988) , 10.1001/JAMA.1988.03410200086031
M Yarus, A specific amino acid binding site composed of RNA Science. ,vol. 240, pp. 1751- 1758 ,(1988) , 10.1126/SCIENCE.3381099
Bharat M. Chowrira, John M. Burke, Binding and cleavage of nucleic acids by the "hairpin" ribozyme Biochemistry. ,vol. 30, pp. 8518- 8522 ,(1991) , 10.1021/BI00099A003
Arthur J. Zaug, Michael D. Been, Thomas R. Cech, The Tetrahymena ribozyme acts like an RNA restriction endonuclease Nature. ,vol. 324, pp. 429- 433 ,(1986) , 10.1038/324429A0