Quantitative determination of free intracellular α-keto acids in neutrophils
作者： Jörg Mühling , Markus Fuchs , Marie E Campos , Jens Gonter , Jörg M Engel
DOI: 10.1016/S1570-0232(03)00163-6
关键词: Fluorescence 、 Neutrophile 、 Quantitative analysis (chemistry) 、 High-performance liquid chromatography 、 Fluorescence spectroscopy 、 Chromatography 、 Intracellular 、 Sample preparation 、 Reversed-phase chromatography 、 Chemistry
摘要: For the first time, a procedure is described for quantitative analysis of free α-keto acid content in human neutrophils (PMNs) relative to single cell number by reversed-phase fluorescence high-performance liquid chromatography. The minimally invasive and unsurpassed quality PMN separation, ease sample preparation as well stability. This method can satisfy rigorous demands an ultra-sensitive, comprehensive rapid intracellular particularly surveillance severe diseases cellular or organ dysfunction.
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