作者: Harry Murakishi , Mark Lesney , Peter Carlson
DOI: 10.1016/B978-0-12-007903-2.50008-8
关键词: In vitro 、 Protoplast 、 Viral replication 、 Cell cycle 、 Cell culture 、 Plant virus 、 Tobacco mosaic virus 、 Virology 、 Biology 、 Virus
摘要: Publisher Summary This chapter focuses on the subject of protoplasts and plant viruses. Protoplasts can be synchronously infected with virus. The presents a valuable alternative means following biochemical cytological events in relation to virus growth cycle precise manner. As animal cell culture, binding has been linked stage replication cycle, as seen TMV tobacco obtained from culture. G1 G2-phase bound more than those other phases. describes current status cultures studies infection, replication, cytopathology, cross-protection, resistance, use vitro methods genetic engineering recover virus-resistant plants.