Ovarian Binding of Intact and Desialylated hCG in Vivo and in Vitro

摘要: The importance of sialic acid residues for combination hCG with ovarian receptor sites was evaluated by comparing the capacities intact and desialylated to compete 125I—labeled binding tissue in vivo vitro. Direct uptake asialohCG also examined For competitive studies, labeled 125I shown be indistinguishable from unlabeled hormone gel nitration, electrophoresis, electrofocusing biological assay. Intravenous administration 200 ng 125I—hCG immature female rats followed maximum 6–12% injected radioactivity at 2 hr, progressive reduction tracer when 100–1200 added before injection. ability reduced 3% activity hormone, consistent rapid clearance consequent low desialylat...