Rapid and precise quantification of HIV-1 RNA in plasma using a branched DNA signal amplification assay.

摘要: The level of human immunodeficiency virus type 1 (HIV-1) RNA in plasma has been quantitated directly with use a solid-phase nucleic acid hybridization assay, based on branched DNA (bDNA) signal amplification technology chemiluminescent detection. Signal is accomplished by the incorporation sites for 1,755 alkaline phosphatase-labeled probes per genome HIV-1, after successive target-specific oligonucleotides and bDNA amplifier molecules. assay performed microwells, much like an immunoassay, amenable to routine laboratory use. Reproducibility specificity studies indicated that method was precise showed no reactivity seronegative donors. HIV-1 levels were 348 seropositive specimens, detection rate 83% those specimens from patients < 500 CD4+ T-cell counts. Plasma found change disease stage, response antiviral therapy. Quantitation HIV-1-infected patients, may be useful monitoring progression therapeutic response.