Combining small molecules for cell reprogramming through an interatomic analysis.

摘要: The knowledge available about the application and generation of induced pluripotent stem cells (iPSC) has grown since their discovery, new techniques to enhance reprogramming process have been described. Among approaches induce iPSC that gained great attention is use small molecules for reprogramming. molecules, unlike genetic manipulation, provides control through shifting concentrations combination different molecules. However, researchers reported "reprogramming cocktails" with variable results drug combinations. Thus, proper successful enhanced a matter discussion. testing all potential combinations in cell lineages very costly time-consuming. Therefore, this article, we discuss already employed generation, followed by systems chemo-biology tools create data sets protein-protein (PPI) chemical-protein (CPI) interaction networks based on used cocktail We further analyzed biological processes associated PPI-CPI provided protein targets be inhibited or expressed In addition, applied interference analysis prospective could negatively affect classical pluripotency-associated factors (SOX2, NANOG, KLF4 OCT4) thus potentially improve protocols.