Determination of amino sugars in environmental samples with high salt content by high-performance anion-exchange chromatography and pulsed amperometric detection.

摘要: Amino sugars were determined in natural samples, including seawater, using high-performance anion-exchange chromatography with pulsed amperometric detection and a new off-line sample cleanup procedure. Samples hydrolyzed 3 M HCl for 5 h (100 °C) neutralized an ion retardation resin. Before injection, salts organic contaminants removed strong cation exchanger the Na+ form. Detection limits amino between 1 4 nM (signal-to-noise ratio 3), allowing first time quantification of seawater without preconcentration. Precision was 2−11% at 20 level. The relatively simple rapid preparation makes it suitable routine analyses.