Telomere shortening in patients with plasma cell disorders.
作者： Alejandra Cottliar , Estela Pedrazzini , Claudia Corrado , María Inés Engelberger , Marina Narbaitz
DOI: 10.1034/J.1600-0609.2003.00157.X
关键词: Monoclonal gammopathy of undetermined significance 、 Internal medicine 、 Fluorescence in situ hybridization 、 Multiple myeloma 、 Cytogenetics 、 Gastroenterology 、 Bone marrow 、 Plasma cell 、 Chromosome instability 、 Molecular biology 、 Telomere 、 Biology
摘要: : Objectives: Telomeres are essential for maintaining chromosomal integrity; their shortening is associated with chromosome instability. The aim of this work was to study telomere length (TL) on bone marrow (BM) cells from patients multiple myeloma (MM) and monoclonal gammopathy undetermined significance (MGUS). Methods: Thirty-one MM patients: 12 at diagnosis (D), 11 relapse (R) eight remission (RE) two cases MGUS were studied. TL based terminal restriction fragment (TRF) assay evaluated. Cytogenetic molecular cytogenetic analyses performed. Telomeric associations (TAs) BM metaphases also studied. Results: TRF analysis in total showed a mean peak value (5.20 ± 0.35 kb) shorter than those observed controls (8.5 ± 0.5 kb) (P < 0.001). Moreover, D R significant (P < 0.001), restored RE. A strong correlation the percentage plasma cell infiltration (BMPCI) (rK = −0.540; P = 0.002) found. Patients abnormal karyotypes (AK) had significantly TRFs that normal (P < 0.05). did not differ respect controls. TA an increased (19.46 ± 1.98%) (6.12 ± 1.87%) (2.00 ± 0.93%) (P < 0.001). Conclusions: reduction (>60% BMPCI AK), suggesting probable association clinical evolution. our findings support idea usually leads frequencies TAs
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