Quantification of Specific Nucleic Acids, Regulated RNA Processing, and Genomic Polymorphisms Using Reversed-Phase HPLC

摘要: The ability to make accurate quantitative measurements of specific nucleic acid molecules is becoming increasingly significant in biomedical science. One important clinical application the quantification viral acids patients’ serum and tissues assess burden for determination disease course efficacy treatment. In this case, accuracy precision measurement are primary importance, as decisions concerning selection medication dose may depend entirely on perceived change load an otherwise asymptomatic patient. A second utility basic research mRNA functionally characterized cell types. These useful because they provide a prediction protein abundance samples which proteins cannot be quantified directly. studies often require microdissection separate interest from neighboring tissues. Again, effective comparison expression multiple genes at different laboratories, under conditions physiological or pharmacological manipulation.