Differential effects of synthetic media on long-term growth, starch accumulation and transcription of ADP-glucosepyrophosphorylase subunit genes in Landoltia punctata.
作者： Chokchai Kittiwongwattana
DOI: 10.1038/S41598-019-51677-W
关键词: Biochemistry 、 Starch 、 Relative growth rate 、 Murashige and Skoog medium 、 Chemistry 、 Gene 、 Gene expression 、 Intergenic region 、 Protein subunit 、 In vitro
摘要: Murashige & Skoog (MS) and Hoagland’s media were previously used for in vitro culture of Landoltia punctata. During subsequent ex culture, the use MS medium resulted a higher growth rate, compared to medium. Thus, starch content L. punctata was hypothesized. Here, strain 5632 isolated characterized using morphological characteristics atpF-atpH intergenic region. early cultivation stage, fresh weight relative rate lower than Conversely, considerably Medium effects on expression genes coding starch-biosynthesis ADP-glucosepyrophosphorylase (AGPase) determined. Genomic fragments small (LeAPS) large (LeAPL1) AGPase subunits characterized. Differential between each subunit observed both media. Additionally, medium, highest correlation coefficients gene found with LeAPS (0.81) followed by LeAPL3 (0.67), LeAPL2 (0.65) LeAPL1 (0.28). In (0.83) (0.62) (0.18) (−0.62). This suggested different levels contributions these biosynthesis
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