Phylogenetic assignment and mechanism of action of a crop growth promoting Rhizobium radiobacter strain used as a biofertiliser on graminaceous crops in Russia

摘要: The taxonomic position of “Agrobacterium radiobacter strain 204,” used in Russia as a cereal crop growth promoting inoculant, was derived by polyphasic approach. phenotypic analyses gave very similar biochemical profiles for 204, Rhizobium NCIMB 9042 (formerly the A. type strain) and R. 13307 Agrobacterium␣tumefaciens strain). High percentage similarities, above species separation level, were observed between 16S rRNA, fusA rpoB housekeeping gene sequences these three strains, genomic DNA–DNA hybridisation 204 against over 70%. Strain is not phytopathogenic it does fix atmospheric N2 or form physical association with roots barley. culture supernatant stimulated rate mobilisation seed reserves barley darkness promoted its shoot light. Gibberellic acid (GA) concentration 1.3 µM but indole acetic undetectable (<50 nM) cultures 204. It concluded that phenotypically genotypically to current mechanism effect on cereals via production plant substances. GA likely play an important role stimulation early