Vascular Network Formation by Human Microvascular Endothelial Cells in Modular Fibrin Microtissues.

摘要: Microvascular endothelial cells (MVEC) are a preferred cell source for autologous revascularization strategies, since they can be harvested and propagated from small tissue biopsies. Biomaterials-based strategies therapeutic delivery of aimed at tailoring the cellular microenvironment to enhance delivery, engraftment, tissue-specific function transplanted cells. In present study, we investigated modular engineering approach using fibrin-based microtissues containing embedded human MVEC fibroblasts (FB). Microtissues were formed water-in-oil emulsion process that produced populations spheroidal modules with diameter 100–200 μm. The formation sprouts within fibrin matrix over 7 days in culture was dependent on presence FB, most robust sprouting occurring 1:3 MVEC:FB ratio. Cell viability high (>90%) significant FB proliferation observed ...