Thiazolidinediones induce osteocyte apoptosis and increase sclerostin expression

摘要: Diabet. Med. 27, 925–932 (2010) Abstract Aims  Thiazolidinediones (TZDs) are associated with a higher risk of bone fracture in women compared men. The aim the present study was to investigate whether TZDs could influence osteocyte behaviour and contribute skeletal phenotype observed TZD-treated patients. Methods  murine MLO-Y4 cell line used as source osteocytes. These cells were cultured for 24 h 0, 10−8 m, 10−7 m, 10−6 m, 10−5 m or 10−4 m pioglitazone, rosiglitazone troglitazone presence absence 17β-oestradiol. extent apoptosis assessed, expression formation inhibitor sclerostin receptor activator nuclear factor κB ligand (RANKL) also. Results  In 17β-oestradiol, induced dose-dependently even at lowest concentration 10−8 m. Furthermore, but not RANKL significantly increased cultures untreated cultures. 17β-oestradiol reduced TZD-induced also up-regulation. Conclusions  findings therefore raise potential concern using post-menopausal where lack oestrogen would prevent up-regulation may aggravate reduction mass these patients.