Spectrophotometric determination of rosmarinic acid in plant cell cultures by complexation with Fe2+ ions
作者： T. L�pez-Arnaldos , M. L�pez-Serrano , A. Ros Barcel� , A. A. Calder�n , J. M. Zapata
DOI: 10.1007/BF00321655
关键词: Stereochemistry 、 Plant cell 、 High-performance liquid chromatography 、 Rosmarinic acid 、 Stoichiometry 、 Lavandula 、 Antioxidant 、 Ion 、 Spectrophotometry 、 Chemistry 、 Chromatography
摘要: A spectrophotometric method for determining rosmarinic acid (α-O-caffeoyl-3,4-dihydroxyphenyllactic acid) in unpurified methanolic extracts from Lavandula cell cultures is reported. It based on a complexation reaction of with Fe2+ to give blue-dark product λmax=572 nm and ɛ573=3.82×103 l mol−1 cm−1. The stoichiometry the optimal conditions colour development are checked. sensitivity accuracy this compared UV-spectral HPLC methods plant cultures; similar results obtained.
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