Asymmetry of the site of choline incorporation into phosphatidylcholine of rat liver microsomes
作者： Joan A. Higgins
DOI: 10.1016/0005-2736(79)90314-6
关键词: Phospholipase C 、 Vesicle 、 Phospholipase 、 Biochemistry 、 Phospholipid 、 Membrane 、 Phosphatidylcholine 、 Bilayer 、 Chemistry 、 Choline 、 Biophysics 、 Cell biology
摘要: [14C]Choline was incorporated into microsomal membranes in vivo, and from CDP-[14C]choline vitro, the site of incorporation determined by hydrolysis outer leaflet membrane bilayer using phospholipase C Clostridium welchii. Labelled phosphatidylcholine found to be concentrated with a specific activity approximately three times that inner leaflet. During CDP-choline treatment vesicles retained labelled-protein contents indicating they remained intact. When microsomes were opened taurocholate after [14C]choline labelled behaved as single pool. Selective intact is not, therefore, consequence specificity C. These results indicate preferentially choline-phosphotransferase pathway this pool phospholipid does not equilibrate
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