Application of Low Coverage Genotyping by Sequencing in Selectively Bred Arctic Charr (Salvelinus alpinus).

摘要: Arctic charr (Salvelinus alpinus) is a species of high economic value for the aquaculture industry, and ecological due to its Holarctic distribution in both marine freshwater environments. Novel genome sequencing approaches enable study population quantitative genetic parameters even on with limited or no prior genomic resources. Low coverage genotyping by (GBS) was applied selected strain Sweden originating from landlocked population. For needs current study, animals year classes 2013 (171 animals, parental population) 2017 (759 animals; 13 full sib families) were used as template identifying wide single nucleotide polymorphisms (SNPs). GBS libraries constructed using PstI MspI restriction enzymes. Approximately 14.5K SNPs passed quality control estimating relationship matrix. Thereafter range analyses conducted order gain insights regarding diversity investigate efficiency information parentage assignment breeding estimation. Heterozygosity estimates suggested slight excess heterozygotes. Furthermore, FST among families class ranged between 0.009 - 0.066. Principal components analysis (PCA) discriminant principal (DAPC) aiming identify existence clusters studied Results obtained accordance pedigree records allowing identification individual families. Additionally, DNA verification performed, results exception putative dam where genotypes potential recording error. Breeding estimation juvenile growth through usage estimated matrix clearly outperformed equivalent terms prediction accuracy (0.51 opposed 0.31). Overall, low has proven be cost-effective platform that expected boost selection program.