High Adhesion and Increased Cell Death Contribute to Strong Biofilm Formation in Klebsiella pneumoniae
作者： Siddhi Desai , Kinjal Sanghrajka , Devarshi Gajjar
关键词: Pathogen 、 Klebsiella 、 Microbiology 、 Chemistry 、 Adhesion 、 Virulence factor 、 Klebsiella pneumoniae 、 Biofilm matrix 、 Matrix (biology) 、 Biofilm
摘要: Klebsiella pneumoniae (Kp), is a frequent cause of hospital and community-acquired infections WHO had declared it as "priority pathogen". Biofilm major virulence factor Kp yet the mechanism strong biofilm formation in unclear. A key objective present study to investigate differences between weak biofilms formed by clinical isolates on various catheters different media conditions identify constituents contributing formation. Quantification matrix components (extracellular DNA (eDNA), protein, exopolysaccharides (EPS), bacterial cells), confocal laser scanning microscopy (CLSM), field emission gun electron (FEG-SEM) flow-cytometry analysis were performed compare matrix. Our results suggest increased latex compared silicone silicone-coated catheters. Higher amounts eDNA, EPS, dead cells observed Kp. High adhesion capacity cell death seem play role biofilms. The enhanced protein appear consequence death.
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