Small-Angle Scattering as a Structural Probe for Nucleic Acid Nanoparticles (NANPs) in a Dynamic Solution Environment.

摘要: Nucleic acid-based technologies are an emerging research focus area for pharmacological and biological studies because they biocompatible can be designed to produce a variety of scaffolds at the nanometer scale. The use nucleic acids (ribonucleic acid (RNA) and/or deoxyribonucleic (DNA)) as building materials in programming assemblies their further functionalization has recently established new exciting field RNA DNA nanotechnology, which have both already produced different functional nanostructures nanodevices. It is evident that resultant architectures require detailed structural characterization technical approaches must employed promote development fields. Small-angle X-ray neutron scattering (SAS) techniques well placed determine conformation nanoparticles (NANPs) under varying solution conditions, thus allowing optimization design. SAS experiments provide information on overall shapes particle dimensions macromolecules ideal following conformational changes molecular ensemble it behaves solution. In addition, inherent differences acids, lipids, proteins, properties isotopes hydrogen, combined with ability uniformly label deuterium, allow one characterize conformations relative dispositions individual components within assembly biomolecules. This article will review application methods summary successful utilization NANP technology date, share our vision its complementing broad suite tools some simulated results never been shared before.