Problems with serum-free production of antithrombin III regarding proteolytic activity and product quality
作者： Markus Teige , Ralf Weidemann , Gerlinde Kretzmer
DOI: 10.1016/0168-1656(94)90171-6
关键词: Microcarrier 、 Biochemistry 、 Immunoassay 、 Recombinant DNA 、 Laboratory flask 、 Antithrombin 、 Cell culture 、 Biology 、 Proteolysis 、 Glycosylation
摘要: Human antithrombin III (AT-III) was produced using a recombinant BHK-21 cell line with microcarrier culture in spinner flasks. Cells were cultivated for the first 4 days medium containing 10% fetal calf serum (FCS). Afterwards, exchanged and production of AT-III occurred at high numbers serum-free medium. The product determined by an immunoassay further analysed after isolation from During cultivation, proteolytic activity detected which caused considerable decomposition. Furthermore, higher level non-glycosylated found production.
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