A preliminary study on the activation and antigen presentation of hepatitis B virus core protein virus-like particle-pulsed bone marrow-derived dendritic cells
作者： Fei-Xiang Ding , Xian Xian , Ying-Jun Guo , Yin Liu , Yue Wang
DOI: 10.1039/C005222A
关键词: Antigen presentation 、 Biology 、 Epitope 、 Western blot 、 Immunogen 、 CD8 、 Annexin A2 、 Cytotoxic T cell 、 Antigen 、 Molecular biology
摘要: Hepatitis B virus core protein virus-like particles (HBc-VLPs) act as a strong immunogen and are suitable for uptake by dendritic cells (DCs), in which they directly promote DC maturation migration. To illustrate the utility of global proteomic analysis techniques elucidating molecular events that altered HBc-VLP-pulsed bone marrow-derived DCs (BMDCs) to gain better understanding mechanisms capture processing BMDCs, an antigen (Ag) delivery system based on BMDCs was developed. Two-dimensional electrophoresis (2-DE) tandem mass spectrometry (MS/MS) analyses were utilized analyze differential expression patterns between untreated BMDCs. Protein spots with significantly levels detected, identified validated. The results showed exogenous HBc-VLPs phagocytosed efficiently enhanced efficacy BMDC Ag presentation, VLPs also induced high Ag-specific CD8+ T displayed cytotoxic lymphocyte (CTL) activity vivo. Several differentially expressed proteins, including growth factor receptor bound 2 (Grb2) annexin A2 (AnxA2), detected analysis, validated western blot.
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