Time-Resolved Fluorescence Energy Transfer Immunoassay

摘要: Time-resolved methods, including both time- and frequency-domain techniques, are of growing importance in fluorescence immunoassay due to their selectivity ability reject background (Soini Hemmila, 1979). Phase methods especially interesting because the instrumentation is simple, can potentially be made compact solid state. We decided test utility variable-frequency phase fluorometry for measurement an antigen-antibody binding reaction, a reaction which could also quantitated by separate method. Thus antibodies antigens were labeled with energy transfer acceptors donors, respectively. This allowed measured from changes donor’s decay kinetics (quenching), or acceptor’s excitation spectrum, caused transfer.