作者: Sara Arana Peña , Nathalia Saraiva Rios , Carmen Mendez Sanchez , Yuliya Lokha , Diego Carballares
DOI: 10.1016/J.IJBIOMAC.2019.10.087
关键词: Layer by layer 、 Substrate (chemistry) 、 Polyethylenimine 、 Layer (electronics) 、 Combinatorial chemistry 、 Chemistry 、 Lipase 、 Glutaraldehyde 、 Immobilized enzyme 、 Candida antarctica 、 Biochemistry 、 Molecular biology 、 Structural biology 、 General Medicine
摘要: This paper shows the step by coimmobilization of up to five different enzymes following two orders in alter effect substrate diffusion limitations. The were lipases A and B from Candida antarctica, Rhizomocur miehei and, Themomyces lanuginosus phospholipase Lecitase Ultra. utilized strategy was a layer immobilization, coating immobilized with polyethylenimine followed crosslinking enzyme PEI glutaraldehyde prevent release, them adding new lipase layer. use previously inactivated biocatalysts (using diethyl p-nitrophenylphosphate) permitted visualize immobilization each layer, which later confirmed SDS-PAGE. also successful complete covalent treated layers. Activity combibiocatalysts using diverse substrates. protocol immobilize an ordered way 5 down-up distribution.