Development of a multiplex PCR for the identification of Staphylococcus genus and four staphylococcal species isolated from food

摘要: Aims:  To develop a multiplex PCR that allows the identification of bacteria belonging to Staphylococcus genus and in particular species xylosus, S. saprophyticus, epidermidis aureus isolated from food manufacturing plants. Methods Results:  Five primer pairs were used PCR, one specific four species. All 31 reference strains yielded product with genus-specific primers. gave fragment corresponding species-specific No amplification Kocuria, Macrococcus Micrococcus was observed our conditions. This performed on 30 Gram-positive cocci different workshops fermented sausages. Among them, 28 belonged 14 identified two epidermidis. Conclusions:  provided reliable repeatable results. It allowed major part isolates, highlighting predominance saprophyticus studied. Significance Impact Study:  tool is useful way screen foodstuff environment monitor these during processing.