A FRET-based two-photon probe for in vivo tracking of pH during a traumatic brain injury process
作者： Baoping Zhai , Shuyang Zhai , Ruilin Hao , Jianjun Xu , Zhihong Liu
DOI: 10.1039/C9NJ04049H
关键词: Aqueous solution 、 Förster resonance energy transfer 、 Two-photon excitation microscopy 、 Fluorophore 、 Traumatic brain injury 、 Fluorescence 、 In vivo 、 Chemistry 、 Biophysics 、 Intracellular
摘要: The traumatic brain injury (TBI) process, a significant cause of neurodegenerative diseases, is generally thought to be accompanied by decrease in pH the intracellular environment. In this work, fluorescence resonance energy transfer (FRET)-based ratiometric two-photon fluorescent probe, FRET-pH, has been designed and fully characterized monitor change so as understand extent TBI process. which nearly 100% efficiency ∼100-fold ratio signal changes, constructed from naphthalene derivative ADN fluorophore SRhB recognition domain for pH. FRET-pH displays both one- responses towards aqueous solution. appropriate pKa value (5.89) supports its use subsequent biological applications. Finally, microscopic imaging living HeLa cells, BV-2 cells rat tissue achieved.
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