Geometrical isomers of violaxanthin in orange juice

摘要: Abstract The geometrical isomers of carotenoids are now attracting considerable interest, due in part to the fact that they known behave differently terms susceptibility oxidation, vitamin A activity and bioavailability. study such has been facilitated by development C 30 stationary phase, which made it possible evaluate their occurrence any source a rapid fashion. In this study, violaxanthin ((3 S ,5 R ,6 ,3′ ,5′ ,6′ )-5,6:5′,6′-diepoxy-5,6,5′,6′-tetrahydro-β,β-carotene-3,3′-diol) fraction orange juice ( Citrus sinensis L. Osbeck) was isolated thin-layer chromatography (TLC) identify different occurring it. Under HPLC conditions described, several were detected, (all- E )-violaxanthin and, above all, (9 Z )-violaxanthin, happened be most important quantitative terms.