Allergy‐related cytokines (IL‐4 and TNF‐α) are induced by Di(2‐ethylhexyl) phthalate and attenuated by plant‐originated glycoprotein (75 kDa) in HMC‐1 cells
作者： Jin Lee , Phil-Sun Oh , Kye-Taek Lim
DOI: 10.1002/TOX.20563
关键词: Endocrinology 、 Interleukin 、 Protein kinase C 、 Protein kinase A 、 Mast cell 、 Degranulation 、 Interleukin 4 、 Tumor necrosis factor alpha 、 Internal medicine 、 Chemistry 、 Molecular biology 、 Histamine
摘要: Phthalate esters as plasticizers have been widespread in the environment and may be associated with development of allergic diseases such asthma atopic dermatitis. In this study, we demonstrated that CTB glycoprotein attenuates reactions caused by di(2-ethylhexyl) phthalate (DEHP) human mast cells (HMC-1). This experiment evaluated degranulation histamine β-hexosaminidase well activities protein kinase C (PKC), stress-activated kinase/c-Jun N-terminal (SAPK/JNK), activator (AP)-1 interleukin (IL)-4 tumor necrosis factor (TNF)-α using immunoblotting reverse transcription-polymerase chain reaction (RT-PCR). Our results revealed presence DEHP inhibits cell, translocation PKC from cytosol to membrane, phosphorylation SAPK/JNK HMC -1 cells. We also found (100 μg mL(-1) ) has suppressive effects on transcriptional activation AP-1, expression IL-4 TNF-α DEHP-treated HMC-1 suggest expressions cytokines
sci-hub.se 参考文章(35)
David M. Neville, Hartmut Glossmann, [9] Molecular weight determination of membrane protein and glycoprotein subunits by discontinuous gel electrophoresis in dodecyl sulfate Methods in Enzymology. ,vol. 32, pp. 92- 102 ,(1974) , 10.1016/0076-6879(74)32012-5
Axel Lorentz, Mikosch Wilke, Gernot Sellge, Hans Worthmann, Jürgen Klempnauer, Michael P. Manns, Stephan C. Bischoff, IL-4-Induced Priming of Human Intestinal Mast Cells for Enhanced Survival and Th2 Cytokine Generation Is Reversible and Associated with Increased Activity of ERK1/2 and c-Fos Journal of Immunology. ,vol. 174, pp. 6751- 6756 ,(2005) , 10.4049/JIMMUNOL.174.11.6751
Ya-Wen Hsu, Kwan-Hwa Chi, Wan-Chen Huang, Wan-Wan Lin, Ceramide inhibits lipopolysaccharide-mediated nitric oxide synthase and cyclooxygenase-2 induction in macrophages: effects on protein kinases and transcription factors. Journal of Immunology. ,vol. 166, pp. 5388- 5397 ,(2001) , 10.4049/JIMMUNOL.166.9.5388
P.H. Howarth, S.T. Holgate, S.J. Wilson, P.G. Bardin, C.H. Heusser, P. Bradding, I.H. Feather, Immunolocalization of cytokines in the nasal mucosa of normal and perennial rhinitic subjects. The mast cell as a source of IL-4, IL-5, and IL-6 in human allergic mucosal inflammation. Journal of Immunology. ,vol. 151, pp. 3853- 3865 ,(1993)
M A Beaven, J Rogers, J P Moore, T R Hesketh, G A Smith, J C Metcalfe, The mechanism of the calcium signal and correlation with histamine release in 2H3 cells. Journal of Biological Chemistry. ,vol. 259, pp. 7129- 7136 ,(1984) , 10.1016/S0021-9258(17)39847-2
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Parkhurst A. Shore, Victor H. Cohn, Alan Burkhalter, A METHOD FOR THE FLUOROMETRIC ASSAY OF HISTAMINE IN TISSUES Journal of Pharmacology and Experimental Therapeutics. ,vol. 127, pp. 182- 186 ,(1959)
Melissa A. Brown, John Hural, Functions of IL-4 and Control of Its Expression Critical Reviews in Immunology. ,vol. 37, pp. 181- 212 ,(2017) , 10.1615/CRITREVIMMUNOL.V37.I2-6.30
Moorthy Palanki, Inhibitors of AP-1 and NF-κB Mediated Transcriptional Activation: Therapeutic Potential in Autoimmune Diseases and Structural Diversity Current Medicinal Chemistry. ,vol. 9, pp. 219- 227 ,(2002) , 10.2174/0929867023371265
Sei-Jung Lee, Kye-Taek Lim, Inhibitory effect of 30-kDa phytoglycoprotein on expression of TNF-α and COX-2 via activation of PKCα and ERK 1/2 in LPS-stimulated RAW 264.7 cells Molecular and Cellular Biochemistry. ,vol. 317, pp. 151- 159 ,(2008) , 10.1007/S11010-008-9843-0