3D Origami-based multifunction-integrated immunodevice: low-cost and multiplexed sandwich chemiluminescence immunoassay on microfluidic paper-based analytical device

摘要: A novel 3D microfluidic paper-based immunodevice, integrated with blood plasma separation from whole samples, automation of rinse steps, and multiplexed CL detections, was developed for the first time based on principle origami (denoted as origami-based device). This device, comprised one test pad surrounded by four folding tabs, could be patterned fabricated wax-printing paper in bulk. In this work, a sandwich-type chemiluminescence (CL) immunoassay introduced into which separate operational procedures several steps including (i) pads above/below (ii) addition reagent/buffer under specific sequence. The behavior, separation, washing protocol, incubation were investigated work. combined typical luminuol-H2O2 system catalyzed Ag nanoparticles, showed excellent analytical performance simultaneous detection tumor markers. samples assayed results obtained agreement reference values parallel single-analyte test. provides new strategy low-cost, sensitive, multiplex point-of-care diagnostics.