Experimental infection of six North American fish species with the North Carolina strain of spring Viremia of Carp Virus

摘要: Abstract Spring viremia of carp virus (SVCV) is a rhabdoviral pathogen associated with disease outbreaks in cultured and wild fish worldwide. Common (Cyprinus carpio carp), koi (C. koi) suffer the highest mortalities from SVCV infections, while other cyprinid species have varying susceptibility. Although salmonid typically are considered refractory to infection by SVCV, there been few reports suggesting has occurred rainbow trout (Oncorhynchus mykiss). There no Percid being infected SVCV. Since first North American outbreak at Carolina farm 2002 eight subsequent detections or among families Cyprinidae Centrarchidae within US Canada. Thus, this exotic potential threat native populations America. We performed multiple experimental challenges three (Salmonidae, Cyprinidae, Percidae) identify risk exposure resident Three species, steelhead mykiss), Chinook salmon (O. tshawytscha), sockeye nerka), were challenged immersion injection isolate. Two fathead minnow (Pimephales promelas) one percid yellow perch (Perca flavescens) also challenged. Koi highly susceptible up 11 months age minnows had chronic expression moderate mortality (29%). induced (33%) fry. Virus cumulative percent ranging 0 100%, fry most vulnerable. A sub-sample survivors screened for plaque assay reverse transcription polymerase chain reaction. In general, all tested positive high viral titers variable persistence overall lower titers. Our suggested that host key factor determining outcome. Other factors, such as broodstock, strain, water temperature, rearing conditions association intrinsic level susceptibility may impact dynamics. This report infecting (yellow perch) family Percidae can similarly primary species.