Microglia depletion in early life programs persistent changes in social, mood-related, and locomotor behavior in male and female rats.

摘要: Microglia, the innate immune cells of central nervous system, regulate brain development by promoting cell genesis, pruning synapses, and removing dying, newly-born or progenitor cells. However, role microglia in early life programming behavior under normal conditions is not well characterized. We used infusion liposomal clodronate to selectively deplete from neonatal rat subsequently assessed impact microglial depletion on juvenile adult motivated behaviors. Liposomal treatment postnatal days one four led greater than 70% loss forebrain day 6 that lasted for approximately ten days. Neonatal reduced anxiety elevated plus maze open field test, increased locomotor activity. On a test social play, decreased chase behaviors relative control animals. There was no change active adults reciprocal interaction but there passive time an number avoidance treated rats controls. overall decrease behavioral despair forced swim neonatally with clodronate. Females, males, showed blunted corticosterone response after acute stress adulthood. These results show are important behavior.